Correlative Light and Electron Microscopy (CLEM) on Biological Samples Using Immuno Electron Microscopy

Application of Ionic Liquid on Biological Samples in Correlative Optical Microscopy and Scanning Electron Microscopy

Correlative optical microscopy and scanning electron microscopy (SEM) combines two techniques enabling the use both optical and electronic analysis techniques on the same sample. For example, optical techniques, such as fluorescence staining, fluorescence in situ hybridization (FISH) could in principle be used with SEM and energy dispersive spectroscopy (EDS) [1]. Optical microscopy provides ef...

microscopy and analysis Correlative Light and Electron Microscopy

D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy Matthew

The processes of life take place in multiple dimensions, but imaging these processes in even three dimensions is challenging. Here we describe a workflow for 3D correlative light and electron microscopy (CLEM) of cell monolayers using fluorescence microscopy to identify and follow biological events, combined with serial blockface scanning electron microscopy to analyse the underlying ultrastruc...

3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy

The processes of life take place in multiple dimensions, but imaging these processes in even three dimensions is challenging. Here, we describe a workflow for 3D correlative light and electron microscopy (CLEM) of cell monolayers using fluorescence microscopy to identify and follow biological events, combined with serial blockface scanning electron microscopy to analyse the underlying ultrastru...

Correlative Light and Electron Microscopy (CLEM) and its applications in infectious disease

Correlative light and electron microscopy (CLEM) is an effective technique used to study biological samples. Signal-specific indicators observable under light microscopy (LM) allow scientists to locate areas of interest for high resolution ultra-structure observation under the electron microscope (EM). Recent method developments provided breakthroughs creating an effective, direct, and accurate...